JOPSS - Search Results

Journal Articles

Immunofluorescence observation of oxidative damage of DNA induced by heavy ions from TIARA

Kitabatake, Satomi*; Ushiroda, Tota*; Hirayama, Ryoichi*; Furusawa, Yoshiya*; Funayama, Tomoo; Yokota, Yuichiro; Okahata, Yoshio*; Ito, Atsushi*

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 86, 2015/03

https://doi.org/10.11484/jaea-review-2014-050

Biological effects of high-LET radiation could be understood in terms of the ion track structure. Therefore the evaluation of the contribution of both core and penumbra regions to biological effects is an important issue for the study of high-LET effects. In the present study, we developed a protocol to make a uniform DNA sheet with insoluble nature in aqueous solution, and explored the applicability to the detection of 8-OHdG distributions after heavy-ion irradiation. Water-insoluble DNA sheet was irradiated with proton and neon ion beams at JAEA-Takasaki. After irradiation DNA samples were incubated with an 8-OHdG antibody followed by with a second antibody containing a fluorescence probe. The preliminary results indicated that upon ion irradiation randomly distributed dot-like fluorescence was observed, suggesting that these dots may be from incident ions.

Journal Articles

Heavy-ion microbeam cell irradiation system at JAERI-Takasaki

Kobayashi, Yasuhiko; Funayama, Tomoo; Wada, Seiichi; Sakashita, Tetsuya; Kakizaki, Takehiko; Hamada, Nobuyuki*; Yokota, Yuichiro; Furusawa, Yoshiya*

KEK Proceedings 2005-5, p.6 - 8, 2005/10

no abstracts in English

Journal Articles

Radiation chemistry research in water with heavy ions

Taguchi, Mitsumasa

Hoshasen Kagaku, (77), p.2 - 7, 2004/05

http://ci.nii.ac.jp/naid/10012966192

no abstracts in English

Journal Articles

System of cell irradiation with a precise number of heavy ions

Kobayashi, Yasuhiko; Funayama, Tomoo; Wada, Seiichi*; Taguchi, Mitsumasa; Watanabe, Hiroshi

Radiation Research, 161(1), p.90 - 91, 2004/01

A single cell irradiation system has been developed for targeting cells individually with a precise number of heavy ions to elucidate radiobiological effects of exactly one particle and to investigate the biological effects of low fluence irradiation with HZE particles. Using the heavy ion microbeam apparatus installed at JAERI-Takasaki, mammalian cells were irradiated in the atmosphere with a single or precise numbers of ions, 13.0 MeV/u 20Ne or 11.5 MeV/u 40Ar. The number of ions traversed the cells attached on the ion track detector CR-39 were counted with a plastic scintillator. Immediately after the irradiation, the position and the number of ion tracks traversed the cell was detected with etching of CR-39 from the opposite side of the cell with alkaline-ethanol solution at 37 $^{circ}$ C. The growths of the cells were observed individually up to 60 hours after irradiation.

Journal Articles

Irradiation of single mammalian cells with a precise number of energetic heavy ions; Applications of microbeams for studying cellular radiation response

Kobayashi, Yasuhiko; Funayama, Tomoo; Wada, Seiichi; Taguchi, Mitsumasa; Watanabe, Hiroshi

Nuclear Instruments and Methods in Physics Research B, 210(1-4), p.308 - 311, 2003/09

A method for detecting the ion hit tracks on the mammalian cultured cells at the irradiation time was established. The cells were attached to the ion track detector CR-39 (100 m thick), then irradiated with 13.0 MeV/u 20Ne or 11.5 MeV/u 40Ar ion beams. Immediately after the irradiation, the cells were refilled with medium, then the CR-39 was etched from the opposite side of the cell with alkaline-ethanol solution at 37 $^{circ}$ C. With the 15 min etching treatment, we obtained the accurate information about the spatial distribution of irradiated ions without significant effect on the cell growth. The continuous observation of the individual cell growth indicated that the growth of ion hit cell was reduced compared with that of non-irradiated one.